Overview

  • Product nameAnti-Glypican 4 antibody
    See all Glypican 4 primary antibodies
  • Description
    Rabbit polyclonal to Glypican 4
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to the C-terminal domain of Human Glypican 4.

  • Positive control
    • Human Liver and Human Pancreas tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab118911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 15 - 20 µg/ml.

Target

Anti-Glypican 4 antibody images

  • ab118911 staining Glypican in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Blue - nuclei (DAPI)

    See Abreview

  • ab118911, at 20 µg/ml, staining Glypican 4 in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry.
  • ab118911, at 20 µg/ml, staining Glypican 4 in Formalin-fixed, Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.

References for Anti-Glypican 4 antibody (ab118911)

ab118911 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Human Tissue sections (human pancreas)
Specification human pancreas
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 17 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Cow Tissue sections (bovine kidney)
Specification bovine kidney
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

Submitted Jan 23 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

Submitted Nov 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"