Overview

  • Product nameAnti-Glypican 4 antibody
    See all Glypican 4 primary antibodies
  • Description
    Rabbit polyclonal to Glypican 4
  • SpecificitySpecific for a core protein epitope (aa220-233) of human Glypican 4.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    TFAQGLAVAG-DVV

    conjugated to KLH, corresponding to amino acids 220-233 of Human Glypican 4

Properties

Applications

Our Abpromise guarantee covers the use of ab52509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 62 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system.
  • Sequence similaritiesBelongs to the glypican family.
  • Cellular localizationCell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ900E8.1 glypican 4 antibody
    • Glypican proteoglycan 4 antibody
    • Gpc4 antibody
    • GPC4_HUMAN antibody
    • K Glypican antibody
    • K-glypican antibody
    • Kglypican antibody
    • OTTHUMP00000024052 antibody
    • Secreted glypican 4 antibody
    • Secreted glypican-4 antibody
    • UNQ474/PRO937 antibody
    see all

Anti-Glypican 4 antibody images

  • IHC image of ab52509 staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52509, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Glypican 4 antibody (ab52509)

This product has been referenced in:
  • Iacobas S  et al. The connexin43-dependent transcriptome during brain development: Importance of genetic background. Brain Res : (2012). Read more (PubMed: 22771707) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"