Overview

  • Product nameAnti-Glypican 5 antibody [EPR6756(B)]
    See all Glypican 5 primary antibodies
  • Description
    Rabbit monoclonal [EPR6756(B)] to Glypican 5
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glypican 5 aa 1-100 (internal sequence).

  • Positive control
    • A549, Human fetal lung, HepG2 and C6 lysates; Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD) KD = 6.98 x 10 -11 M Learn more about KD
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberEPR6756(B)
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 64 kDa).
IHC-P 1/250 - 1/500.
  • Application notesIs unsuitable for ICC/IF.
  • Target

    Anti-Glypican 5 antibody [EPR6756(B)] images

    • All lanes : Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified)

      Lane 1 : C6 whole cell lysate
      Lane 2 : Mouse spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 64 kDa
      Observed band size : 64 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified) + Human fetal lung at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 64 kDa
      Observed band size : 64 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified) + A549 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 64 kDa
      Observed band size : 64 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded human glioma with purified ab124886 at a working dilution of 1/100. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • All lanes : Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (Unpurified)

      Lane 1 : A549 cell lysate
      Lane 2 : Human fetal lung lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 64 kDa
      Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    • Unpurified ab124886, at 1/250 dilution, staining Glypican 5 in paraffin-embedded Human brain tissue by Immunohistochemistry.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)

    This product has been referenced in:
    • Takeuchi M  et al. Transcriptional Dynamics of Immortalized Human Mesenchymal Stem Cells during Transformation. PLoS One 10:e0126562 (2015). WB ; Human . Read more (PubMed: 25978455) »
    • Dhir A  et al. Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs. Nat Struct Mol Biol 22:319-27 (2015). WB ; Human . Read more (PubMed: 25730776) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"