Overview

  • Product name
  • Description
    Rabbit polyclonal to Glypican 6
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Glypican 6 aa 503-536 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9Y625

  • Positive control
    • Human kidney tissue, 293 cells and Glypican 6-transfected 293 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/50 - 1/100. Predicted molecular weight: 63 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases (By similarity). Enhances migration and invasion of cancer cells through WNT5A signaling.
  • Tissue specificity
    Widely expressed. High expression in fetal kidney and lung and lower expressions in fetal liver and brain. In adult tissues, very abundant in ovary, high levels also observed in liver, kidney, small intestine and colon. Not detected in peripheral blood leukocytes. Detected in breast cancer cells (at protein level).
  • Involvement in disease
    Defects in GPC6 are a cause of omodysplasia type 1 (OMOD1) [MIM:258315]. OMOD1 is a rare autosomal recessive skeletal dysplasia characterized by severe congenital micromelia with shortening and distal tapering of the humeri and femora to give a club-like appearance. Typical facial features include a prominent forehead, frontal bossing, short nose with a depressed broad bridge, short columella, anteverted nostrils, long philtrum, and small chin. Note=Point mutations leading to protein truncation, as well as larger genomic rearrangements resulting in exon deletions, have been found in family segregating omodysplasia type 1. All mutations identified in individuals affected by omodysplasia could lead to the absence of a functional protein, the mutant RNAs being suspected to be nonsense-mediated mRNA decay (NMD) targets. Even if the mRNA escapes NMD and is translated, all mutations are expected to disrupt the three-dimensional protein structure and often to abolish multiple highly conserved cysteine residues.
  • Sequence similarities
    Belongs to the glypican family.
  • Cellular localization
    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • GPC 6 antibody
    • Glypican 6 [Precursor] antibody
    • Glypican proteoglycan 6 antibody
    • Gpc6 antibody
    • GPC6_HUMAN antibody
    • MGC126288 antibody
    • OMIMD1 antibody
    • PRO705 antibody
    • Secreted glypican 6 antibody
    • Secreted glypican-6 antibody
    • UNQ369 antibody
    see all

Images

  • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling Glypican 6 using ab170523 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of 293 cells, labeling Glypican 6 using ab170523 at a 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • All lanes : Anti-Glypican 6 antibody (ab170523) at 1/50 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : Glypican 6-transfected 293 cell lysate

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 63 kDa

References

ab170523 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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