Overview

  • Product nameGM-CSF Rat ELISA KitSee all GM-CSF kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    12.35 pg/ml - 3000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 90.53 81% - 101%
    Serum 136.4 128% - 143%
    Plasma 118.8 88% - 136%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s GM-CSF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat GM-CSF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for rat GM-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti- rat GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceGM-CSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of GM-CSF is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
  • Cellular localizationSecreted
  • Alternative names
    • Colony stimulating factor
    • Colony stimulating factor 2 (granulocyte macrophage)
    • CSF
    • CSF 2
    • CSF2
    • GMCSF
    • Granulocyte macrophage colony stimulating factor
    • MGC131935
    • MGC138897
    • Molgramostin
    • Sargramostim
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

GM-CSF Rat ELISA Kit images

  • Representative standard curve using ab100762

  • Representative standard curve using ab100762

Protocols

References for GM-CSF Rat ELISA Kit (ab100762)

ab100762 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"