Anti-GM130 antibody - cis-Golgi Marker (ab31561)

Overview

  • Product nameAnti-GM130 antibody - cis-Golgi Marker
    See all GM130 primary antibodies
  • Description
    Rabbit polyclonal to GM130 - cis-Golgi Marker
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from within residues 950 to the C-terminus of Rat GM130.

  • Positive control
    • This antibody gave a positive signal in the following Human whole cell lysates: HeLa; MCF7.

Properties

Applications

Our Abpromise guarantee covers the use of ab31561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 111 kDa).

Target

  • FunctionGolgi auto-antigen; probably involved in maintaining cis-Golgi structure.
  • Sequence similaritiesBelongs to the GOLGA2 family.
  • DomainExtended rod-like protein with coiled-coil domains.
  • Cellular localizationGolgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 130 kDa cis Golgi matrix protein antibody
    • 130 kDa cis-Golgi matrix protein antibody
    • Cis golgi matrix protein GM130 antibody
    • GM130 antibody
    • Gm130 autoantigen antibody
    • GOGA2_HUMAN antibody
    • GOLGA 2 antibody
    • Golga2 antibody
    • Golgi autoantigen antibody
    • Golgi autoantigen golgin subfamily a 2 antibody
    • Golgi matrix protein GM130 antibody
    • Golgin 95 antibody
    • golgin A2 antibody
    • Golgin subfamily a 2 antibody
    • Golgin subfamily A member 2 antibody
    • Golgin-95 antibody
    • MGC20672 antibody
    • SY11 protein antibody
    see all

Anti-GM130 antibody - cis-Golgi Marker images

  • ICC/IF image of ab31561 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab31561, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • All lanes : Anti-GM130 antibody - cis-Golgi Marker (ab31561) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 111 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

References for Anti-GM130 antibody - cis-Golgi Marker (ab31561)

This product has been referenced in:
  • Maxfield KE  et al. Comprehensive functional characterization of cancer-testis antigens defines obligate participation in multiple hallmarks of cancer. Nat Commun 6:8840 (2015). Read more (PubMed: 26567849) »

See 1 Publication for this product

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Application Western blot
Sample Human Cell lysate - whole cell (HeLa HEK293 NIH-3T3 PC12)
Loading amount 30 µg
Specification HeLa HEK293 NIH-3T3 PC12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Oct 28 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"