Overview

  • Product nameAnti-GM130 antibody [EP818Y]
    See all GM130 primary antibodies
  • Description
    Rabbit monoclonal [EP818Y] to GM130
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GM130. The exact sequence is proprietary.

  • Positive control
    • WB: HeLa cell lysate. ICC/IF: MCF-7 cells. Flow Cyt: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab76154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 112 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionGolgi auto-antigen; probably involved in maintaining cis-Golgi structure.
    • Sequence similaritiesBelongs to the GOLGA2 family.
    • DomainExtended rod-like protein with coiled-coil domains.
    • Cellular localizationGolgi apparatus > Golgi stack membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 130 kDa cis Golgi matrix protein antibody
      • 130 kDa cis-Golgi matrix protein antibody
      • Cis golgi matrix protein GM130 antibody
      • GM130 antibody
      • Gm130 autoantigen antibody
      • GOGA2_HUMAN antibody
      • GOLGA 2 antibody
      • Golga2 antibody
      • Golgi autoantigen antibody
      • Golgi autoantigen golgin subfamily a 2 antibody
      • Golgi matrix protein GM130 antibody
      • Golgin 95 antibody
      • golgin A2 antibody
      • Golgin subfamily a 2 antibody
      • Golgin subfamily A member 2 antibody
      • Golgin-95 antibody
      • MGC20672 antibody
      • SY11 protein antibody
      see all

    Anti-GM130 antibody [EP818Y] images

    • Anti-GM130 antibody [EP818Y] (ab76154) at 1/5000 dilution (purified) + HeLa whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

      Predicted band size : 112 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)
      Blocking and dilution buffer: 5% NFDM/TBST
    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling GM130 with purified ab76154 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • Flow Cytometry analysis of HeLa cells labelling GM130 with purified ab76154 at a dilution of 1/40 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • Anti-GM130 antibody [EP818Y] (ab76154) at 1/1000 dilution (unpurified) + HeLa cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 112 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)

    References for Anti-GM130 antibody [EP818Y] (ab76154)

    ab76154 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76154.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"