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A synthetic peptide corresponding to residues near the N-term of human GM130.
Produced under U.S. Patent No. 5,675,063.
This product is available conjugated to DyLight® 488 see ab139860.
Our Abpromise guarantee covers the use of ab52649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250. PFA fixation should be most suitable.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Overnight incubation is recommended.|
|IHC-Fr||Use at an assay dependent dilution.|
|WB||1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 112 kDa).|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"