RabMAb

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

Overview

  • Product nameAnti-GM130 antibody [EP892Y] - cis-Golgi Marker
    See all GM130 primary antibodies
  • Description
    Rabbit monoclonal [EP892Y] to GM130 - cis-Golgi Marker
  • SpecificityMouse and rat cell lines pc12, 3t3, raw 264.7 were tested positive in WB. However, brain, kidney, spleen and heart were negative from the two species.
  • Tested applicationsICC/IF, IHC-P, ICC, IHC-Fr, IHC, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human, Monkey, African Green Monkey
  • Immunogen

    A synthetic peptide corresponding to residues near the N-term of human GM130.

  • Positive control
    • Hela cell lysate and human liver tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-GM130 antibody (Alexa Fluor® 488) - cis-Golgi Marker [EP892Y] (ab195302)

    Anti-GM130 antibody (Alexa Fluor® 647) - cis-Golgi Marker [EP892Y] (ab195303)

Properties

Applications

Our Abpromise guarantee covers the use of ab52649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250. PFA fixation should be most suitable.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Overnight incubation is recommended.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC Use at an assay dependent concentration. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 112 kDa).
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionGolgi auto-antigen; probably involved in maintaining cis-Golgi structure.
    • Sequence similaritiesBelongs to the GOLGA2 family.
    • DomainExtended rod-like protein with coiled-coil domains.
    • Cellular localizationGolgi apparatus > Golgi stack membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 130 kDa cis Golgi matrix protein antibody
      • 130 kDa cis-Golgi matrix protein antibody
      • Cis golgi matrix protein GM130 antibody
      • GM130 antibody
      • GM130 autoantigen antibody
      • GOGA2_HUMAN antibody
      • GOLGA 2 antibody
      • GOLGA2 antibody
      • Golgi autoantigen antibody
      • Golgi autoantigen golgin subfamily a 2 antibody
      • Golgi matrix protein GM130 antibody
      • Golgin 95 antibody
      • golgin A2 antibody
      • Golgin subfamily a 2 antibody
      • Golgin subfamily A member 2 antibody
      • Golgin-95 antibody
      • MGC20672 antibody
      • SY11 protein antibody
      see all

    Anti-GM130 antibody [EP892Y] - cis-Golgi Marker images

    • Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/200000 dilution + 10 µg of HeLa cell lysate

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 112 kDa
    • ab52649 (1/500) staining GM130 in paraffin-embedded Human liver tissue sections.ab52649 (1/500) staining GM130 in paraffin-embedded Human liver tissue sections.
    • ab52649 staining GM130 (magenta) in monkey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody. Nuclei stained with Picogreen.

      See Abreview

    • ab52649 staining GM130 in human ARPE-19 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. The sample was incubated with the primary antibody (1/500 in 1% goat serum, 0.1%TX100, 1 x PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary.

      See Abreview

    • ab52649 staining GM130 in Bovine brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 5% BSA for 90 minutes at 37°C. Samples were incubated with primary antibody (1/100 in 0.1% saponin + 1% BSA ) for 18 hours at 4°C. An undiluted Alexa Fluor®568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • ICC/IF image of ab52946 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52946, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
    • ICC/IF image of ab52649 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52649, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    This product has been referenced in:
    • Mitsuda S  et al. Ursolic acid, a natural pentacyclic triterpenoid, inhibits intracellular trafficking of proteins and induces accumulation of intercellular adhesion molecule-1 linked to high-mannose-type glycans in the endoplasmic reticulum. FEBS Open Bio 4:229-39 (2014). ICC/IF ; Human . Read more (PubMed: 24649404) »
    • Hofmann K  et al. A novel alkyne cholesterol to trace cellular cholesterol metabolism and localization. J Lipid Res 55:583-91 (2014). Read more (PubMed: 24334219) »

    See all 18 Publications for this product

    Product Wall

    Application Immunoprecipitation
    Sample Rat Tissue lysate - whole (kidney)
    Total protein in input 500 µg
    Immuno-precipitation step Protein G
    Specification kidney
    Username

    Gustavo Frindt

    Verified customer

    Submitted Jul 02 2015

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (4-15% SDS-PAGE)
    Sample Human Cell lysate - whole cell (HeLa)
    Specification HeLa
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Armen Petrosyan

    Verified customer

    Submitted Nov 19 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: rt°C
    Sample Mouse Cell (nih3t3)
    Specification nih3t3
    Permeabilization Yes - triton X100 0.5%
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 31 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Rat Cell (Rat hepatocytes)
    Specification Rat hepatocytes
    Permeabilization No
    Fixative Formaldehyde
    Username

    Dr. Armen Petrosyan

    Verified customer

    Submitted Oct 28 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step 3% BSA and 0.5% TX100 as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample Monkey Cell (Kidney)
    Specification Kidney
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Dr. Aaron Halpen

    Verified customer

    Submitted Sep 29 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample Mouse Cell (hepatocytes)
    Specification hepatocytes
    Permeabilization Yes - 0.1% Triton x-100
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 26 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 80 µg
    Gel Running Conditions Reduced Denaturing (8% gel)
    Sample Mouse Cell lysate - whole cell (liver cells)
    Specification liver cells
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 26 2013

    Application Immunocytochemistry/ Immunofluorescence
    Sample Monkey Cell (COS cells)
    Specification COS cells
    Fixative Formaldehyde
    Permeabilization Yes - 0.1% TritonX-100
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 09 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (White blood cells)
    Specification White blood cells
    Fixative Formaldehyde
    Permeabilization Yes - 0.1% TritonX-100
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 05 2013

    Thank you for your enquiry.

    I would like to reassure you that both these antibodies are covered by our 6 month guarantee for WB and for human samples.

    For more details regarding our guarantee, please see the following page from ou...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"