RabMAb

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

Overview

  • Product nameAnti-GM130 antibody [EP892Y] - cis-Golgi Marker
    See all GM130 primary antibodies
  • Description
    Rabbit monoclonal [EP892Y] to GM130 - cis-Golgi Marker
  • SpecificityMouse and rat cell lines pc12, 3t3, raw 264.7 were tested positive in WB. However, brain, kidney, spleen and heart were negative from the two species.
  • Tested applicationsICC/IF, IHC-P, ICC, IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human, Monkey, African Green Monkey
  • Immunogen

    A synthetic peptide corresponding to residues near the N-term of human GM130.

  • Positive control
    • Hela cell lysate and human liver tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    This product is available conjugated to DyLight® 488 see ab139860.

Properties

Applications

Our Abpromise guarantee covers the use of ab52649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250. PFA fixation should be most suitable.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Overnight incubation is recommended.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 112 kDa).
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionGolgi auto-antigen; probably involved in maintaining cis-Golgi structure.
    • Sequence similaritiesBelongs to the GOLGA2 family.
    • DomainExtended rod-like protein with coiled-coil domains.
    • Cellular localizationGolgi apparatus > Golgi stack membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 130 kDa cis Golgi matrix protein antibody
      • 130 kDa cis-Golgi matrix protein antibody
      • Cis golgi matrix protein GM130 antibody
      • GM130 antibody
      • GM130 autoantigen antibody
      • GOGA2_HUMAN antibody
      • GOLGA 2 antibody
      • GOLGA2 antibody
      • Golgi autoantigen antibody
      • Golgi autoantigen golgin subfamily a 2 antibody
      • Golgi matrix protein GM130 antibody
      • Golgin 95 antibody
      • golgin A2 antibody
      • Golgin subfamily a 2 antibody
      • Golgin subfamily A member 2 antibody
      • Golgin-95 antibody
      • MGC20672 antibody
      • SY11 protein antibody
      see all

    Anti-GM130 antibody [EP892Y] - cis-Golgi Marker images

    • Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/200000 dilution + 10 µg of HeLa cell lysate

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 112 kDa
    • ab52649 (1/500) staining GM130 in paraffin-embedded Human liver tissue sections.ab52649 (1/500) staining GM130 in paraffin-embedded Human liver tissue sections.
    • ab52649 staining GM130 (magenta) in monkey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody. Nuclei stained with Picogreen.

      See Abreview

    • ab52649 staining GM130 in human ARPE-19 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. The sample was incubated with the primary antibody (1/500 in 1% goat serum, 0.1%TX100, 1 x PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary.

      See Abreview

    • ab52649 staining GM130 in Bovine brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 5% BSA for 90 minutes at 37°C. Samples were incubated with primary antibody (1/100 in 0.1% saponin + 1% BSA ) for 18 hours at 4°C. An undiluted Alexa Fluor®568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • ICC/IF image of ab52946 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52946, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
    • ICC/IF image of ab52649 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52649, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    This product has been referenced in:
    • Mitsuda S  et al. Ursolic acid, a natural pentacyclic triterpenoid, inhibits intracellular trafficking of proteins and induces accumulation of intercellular adhesion molecule-1 linked to high-mannose-type glycans in the endoplasmic reticulum. FEBS Open Bio 4:229-39 (2014). ICC/IF ; Human . Read more (PubMed: 24649404) »
    • Hofmann K  et al. A novel alkyne cholesterol to trace cellular cholesterol metabolism and localization. J Lipid Res 55:583-91 (2014). Read more (PubMed: 24334219) »

    See all 13 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: rt°C
    Sample Mouse Cell (nih3t3)
    Specification nih3t3
    Permeabilization Yes - triton X100 0.5%
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 31 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Rat Cell (Rat hepatocytes)
    Specification Rat hepatocytes
    Permeabilization No
    Fixative Formaldehyde
    Username

    Dr. Armen Petrosyan

    Verified customer

    Submitted Oct 28 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step 3% BSA and 0.5% TX100 as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample Monkey Cell (Kidney)
    Specification Kidney
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Dr. Aaron Halpen

    Verified customer

    Submitted Sep 29 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample Mouse Cell (hepatocytes)
    Specification hepatocytes
    Permeabilization Yes - 0.1% Triton x-100
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 26 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 80 µg
    Gel Running Conditions Reduced Denaturing (8% gel)
    Sample Mouse Cell lysate - whole cell (liver cells)
    Specification liver cells
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 26 2013

    Application Immunocytochemistry/ Immunofluorescence
    Sample Monkey Cell (COS cells)
    Specification COS cells
    Fixative Formaldehyde
    Permeabilization Yes - 0.1% TritonX-100
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 09 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (White blood cells)
    Specification White blood cells
    Fixative Formaldehyde
    Permeabilization Yes - 0.1% TritonX-100
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 05 2013

    Thank you for your enquiry.

    I would like to reassure you that both these antibodies are covered by our 6 month guarantee for WB and for human samples.

    For more details regarding our guarantee, please see the following page from ou...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (testes)
    Specification testes
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.03% Triton X 100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 07 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (A549)
    Specification A549
    Fixative Formaldehyde
    Permeabilization No
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Username

    Dr. Armen Petrosyan

    Verified customer

    Submitted Dec 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"