RabMAb

Anti-GM130 [EP892Y] antibody - cis-Golgi Marker (ab52649)

Overview

  • Product nameAnti-GM130 [EP892Y] antibody - cis-Golgi MarkerSee all GM130 primary antibodies ...
  • Description
    Rabbit monoclonal [EP892Y] to GM130 - cis-Golgi Marker
  • SpecificityMouse and rat cell lines pc12, 3t3, raw 264.7 were tested positive in WB. However, brain, kidney, spleen and heart were negative from the two species.
  • Tested applicationsICC/IF, IHC-P, IHC-Fr, WB, IP more details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human, African Green Monkey
  • Immunogen

    A synthetic peptide corresponding to residues near the N-term of human GM130.

  • Positive control
    • Hela cell lysate and human liver tissue.
  • General notes

    Produced under U.S. Patent No. 5,675,063.

    This product is available conjugated to DyLight® 488 see ab139860.

Properties

Applications

Our Abpromise guarantee covers the use of ab52649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF 1/100 - 1/250. PFA fixation should be most suitable.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Overnight incubation is recommended.
IHC-Fr Use at an assay dependent dilution.
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 112 kDa).
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionGolgi auto-antigen; probably involved in maintaining cis-Golgi structure.
    • Sequence similaritiesBelongs to the GOLGA2 family.
    • DomainExtended rod-like protein with coiled-coil domains.
    • Cellular localizationGolgi apparatus > Golgi stack membrane.
    • Target information above from: UniProt accession Q08379 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • 130 kDa cis Golgi matrix protein antibody
      • 130 kDa cis-Golgi matrix protein antibody
      • Cis golgi matrix protein GM130 antibody
      • GM130 antibody
      • GM130 autoantigen antibody
      • GOGA2_HUMAN antibody
      • GOLGA 2 antibody
      • GOLGA2 antibody
      • Golgi autoantigen antibody
      • Golgi autoantigen golgin subfamily a 2 antibody
      • Golgi matrix protein GM130 antibody
      • Golgin 95 antibody
      • golgin A2 antibody
      • Golgin subfamily a 2 antibody
      • Golgin subfamily A member 2 antibody
      • Golgin-95 antibody
      • MGC20672 antibody
      • SY11 protein antibody
      see all

    Anti-GM130 [EP892Y] antibody - cis-Golgi Marker images

    • Anti-GM130 [EP892Y] antibody - cis-Golgi Marker (ab52649) at 1/200000 dilution + 10 µg of HeLa cell lysate

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 112 kDa
    • ab52649 (1/500) staining GM130 in paraffin-embedded Human liver tissue sections.
    • ab52649 staining GM130 in human ARPE-19 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. The sample was incubated with the primary antibody (1/500 in 1% goat serum, 0.1%TX100, 1 x PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary.

      See Abreview

    • ab52649 staining GM130 in Bovine brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 5% BSA for 90 minutes at 37°C. Samples were incubated with primary antibody (1/100 in 0.1% saponin + 1% BSA ) for 18 hours at 4°C. An undiluted Alexa Fluor®568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • ICC/IF image of ab52946 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52946, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
    • ICC/IF image of ab52649 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52649, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-GM130 [EP892Y] antibody - cis-Golgi Marker (ab52649)

    This product has been referenced in:
    • Haataja L  et al. Proinsulin intermolecular interactions during secretory trafficking in pancreatic ß cells. J Biol Chem 288:1896-906 (2013). IHC-P ; Mouse . Read more (PubMed: 23223446) »
    • Rogers RS  et al. Proteomics analyses of human optic nerve head astrocytes following biomechanical strain. Mol Cell Proteomics 11:M111.012302 (2012). WB ; Human . Read more (PubMed: 22126795) »

    See all 8 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 80 µg
    Gel Running Conditions Reduced Denaturing (8% gel)
    Sample Mouse Cell lysate - whole cell (liver cells)
    Specification liver cells
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 26 2013

    Application Immunocytochemistry/ Immunofluorescence
    Sample Monkey Cell (COS cells)
    Specification COS cells
    Fixative Formaldehyde
    Permeabilization Yes - 0.1% TritonX-100
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 09 2013

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (White blood cells)
    Specification White blood cells
    Fixative Formaldehyde
    Permeabilization Yes - 0.1% TritonX-100
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 05 2013

    Thank you for your enquiry.

    I would like to reassure you that both these antibodies are covered by our 6 month guarantee for WB and for human samples.

    For more details regarding our guarantee, please see the following page from ou...

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    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (testes)
    Specification testes
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.03% Triton X 100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 07 2013

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (A549)
    Specification A549
    Fixative Formaldehyde
    Permeabilization No
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Username

    Mr. Armen Petrosyan

    Verified customer

    Submitted Dec 03 2012

    Thank you for contacting us.

    I can confirm that we are pleased to serve customer requests if possible. I would need more information about the amount and concentration that is needed. As soon as I get this information I will contact the lab fo...

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    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (chondrocyte cell line ATDC5)
    Specification chondrocyte cell line ATDC5
    Fixative Paraformaldehyde
    Permeabilization Yes - Triton X-100 0.5%
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 29 2012

    Application Immunocytochemistry/ Immunofluorescence
    Sample African Green Monkey Cell (COS7)
    Specification COS7
    Fixative Formaldehyde
    Permeabilization Yes - 0.2% Triton
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 18 2012

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Cultured Hippocamp Neurons)
    Specification Cultured Hippocamp Neurons
    Fixative Formaldehyde
    Permeabilization Yes - 0.2% Triton
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"