Anti-GMP Synthase antibody (ab87249)

Overview

  • Product nameAnti-GMP Synthase antibody
    See all GMP Synthase primary antibodies
  • Description
    Rabbit polyclonal to GMP Synthase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 252-301 (CTALLNRALN QEQVIAVHID NGFMRKRESQ SVEEALKKLG IQVKVINAAH) of Human GMP Synthase, NP_003866

  • Positive control
    • MCF7 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInvolved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division.
  • PathwayPurine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.
  • Involvement in diseaseNote=A chromosomal aberration involving GMPS is found in acute myeloid leukemias. Translocation t(3,11)(q25,q23) with MLL.
  • Sequence similaritiesContains 1 glutamine amidotransferase type-1 domain.
    Contains 1 GMP-binding domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glutamine amidotransferase antibody
    • GMP synthase [glutamine hydrolyzing] antibody
    • GMP synthase [glutamine-hydrolyzing] antibody
    • GMP synthetase antibody
    • Gmps antibody
    • GUAA_HUMAN antibody
    • Guanine monphosphate synthetase antibody
    • Guanosine 5' monophosphate synthase antibody
    • MLL/GMPS fusion protein antibody
    see all

Anti-GMP Synthase antibody images

  • Anti-GMP Synthase antibody (ab87249) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 77 kDa

References for Anti-GMP Synthase antibody (ab87249)

ab87249 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Xenopus laevis Cell lysate - whole cell (egg extract (total and chromatin))
Specification egg extract (total and chromatin)
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Sep 03 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"