Anti-GNAL antibody (ab74049)
Key features and details
- Rabbit polyclonal to GNAL
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GNAL antibody -
Description
Rabbit polyclonal to GNAL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human GNAL aa 60-90 (internal sequence).
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Positive control
- Human brain tissue. Extracts from LOVO cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters. -
Tissue specificity
Detected in olfactory neuroepithelium, brain, testis, and to a lower extent in retina, lung alveoli, spleen. Trace amounts where seen in kidney, adrenal gland and liver. Found to be expressed in all the insulinomas examined. -
Sequence similarities
Belongs to the G-alpha family. G(s) subfamily. - Information by UniProt
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Database links
- Entrez Gene: 2774 Human
- Entrez Gene: 14680 Mouse
- Entrez Gene: 24611 Rat
- Omim: 139312 Human
- SwissProt: P38405 Human
- SwissProt: Q8CGK7 Mouse
- SwissProt: P38406 Rat
- Unigene: 136295 Human
see all -
Alternative names
- Adenylate cyclase stimulating G alpha protein, olfactory type antibody
- Adenylate cyclase-stimulating G alpha protein antibody
- DYT25 antibody
see all
Images
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All lanes : Anti-GNAL antibody (ab74049) at 1/500 dilution
Lane 1 : extracts from LOVO cells
Lane 2 : extracts from LOVO cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa -
Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab74049, in the absence (left) or presence (right) of the immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab74049 has been referenced in 4 publications.
- Song H et al. Quantitative Proteomic Study Reveals Up-Regulation of cAMP Signaling Pathway-Related Proteins in Mild Traumatic Brain Injury. J Proteome Res 17:858-869 (2018). PubMed: 29215295
- Gonçalves I et al. 'Smelling' the cerebrospinal fluid: olfactory signaling molecules are expressed in and mediate chemosensory signaling from the choroid plexus. FEBS J 283:1748-66 (2016). PubMed: 26934374
- Zhang L et al. Dopamine receptor and Ga(olf) expression in DYT1 dystonia mouse models during postnatal development. PLoS One 10:e0123104 (2015). WB . PubMed: 25860259
- Griffin CA et al. MOR23 promotes muscle regeneration and regulates cell adhesion and migration. Dev Cell 17:649-61 (2009). WB ; Mouse . PubMed: 19922870