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Western blot - GNAQ antibody (ab75825)
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Immunocytochemistry/ Immunofluorescence - GNAQ antibody (ab75825)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GNAQ antibody (ab75825)
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Product Name
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GNAQ antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GNAQ
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GNAQ. (Peptide available as ab86404.)
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Dog, Pig, Xl
(Due to sequence homology)
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Specificity
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The immunogen has 66% homology with GNA11
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following lysates:
HepG2 Whole Cell; Mouse Pancreas Tissue; Mouse Kidney Tissue; Rat Liver Tissue.
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Cellular localization
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Cytoplasmic and Plasma membrane
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Research areas
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Cardiovascular >> Heart >> Apoptosis
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> G Proteins
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> GTP
Neuroscience >> Neurology process >> Neural Signal Transduction
Cardiovascular >> Blood >> Coagulation >> Regulatory
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Relevance
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GNAQ belong to the family of heterotrimeric Guanine nucleotide-binding proteins proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. GNAQ is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta. in recent studies over expression of GNAQ is associated with cardiomyocyte apoptosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.900 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GNAQ antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GNAQ antibody images, references, products related to ab75825 and other tools.
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GNAQ antibody images:
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 Western blot - GNAQ antibody (ab75825)
All lanes : GNAQ antibody (ab75825) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lane 4 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP)
developed using the ECL technique
Predicted band size : 42 kDa
Observed band size : 42 kDa
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - GNAQ antibody (ab75825)
ICC/IF image of ab75825 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75825, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GNAQ antibody (ab75825)
IHC image of G protein alpha q staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75825, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Search PubMed (MEDLINE) for references to GNAQ
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GNAQ antibody - more information
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Customer reviews (feedback) regarding GNAQ antibody |
Customer FAQs regarding GNAQ antibody |
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