Overview

  • Product name
    Anti-GNAQ + GNA11 antibody
  • Description
    Rabbit polyclonal to GNAQ + GNA11
  • Specificity
    Specific for GNAQ and GNA11. It does not recognize other non-pertussis toxin-sensitive G proteins.
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammal
  • Immunogen

    Synthetic peptide:

    QLNLKEYNLV

    conjugated to KLH, corresponding to amino acids 350-359 of Human GNA11 or 344-353 of Human GNAQ and GNA11.

  • Positive control
    • NIH-3T3 cell membrane extract. Rat brain microsomal preparation.

Properties

Applications

Our Abpromise guarantee covers the use of ab79337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB 1/500 - 1/2000. Predicted molecular weight: 42 kDa.

Target

  • Relevance
    Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. GNAQ and GNA11 are alpha subunits of one of the heterotrimeric GTP-binding proteins and mediate stimulation of phospholipase C.
  • Cellular localization
    Cytoplasm. Plasma membrane.
  • Database links
  • Alternative names
    • G alpha 11 antibody
    • G alpha q antibody
    • G protein subunit alpha 11 antibody
    • GAQ antibody
    • GNA 11 antibody
    • GNA11 antibody
    • GNAQ antibody
    • Guanine nucleotide binding protein (G protein) alpha 11 (Gq class) antibody
    • Guanine nucleotide binding protein (G protein) alpha 11 antibody
    • Guanine nucleotide binding protein (G protein) q polypeptide antibody
    • Guanine nucleotide binding protein alpha q antibody
    • Guanine nucleotide binding protein G(q) subunit alpha antibody
    • Guanine nucleotide binding protein G(y) subunit alpha antibody
    • Guanine nucleotide binding protein Gq class GNA11 antibody
    • Guanine nucleotide binding protein subunit alpha 11 antibody
    see all

Images

  • Anti-GNAQ + GNA11 antibody (ab79337) at 1/1000 dilution + NIH-3T3 cell membrane extract at 20 µg

    Secondary
    Goat anti-Rabbit HRP-conjugated IgG at 1/5000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • Anti-GNAQ + GNA11 antibody (ab79337) at 1/2000 dilution + Rat brain microsomal preparation at 20 µg

    Secondary
    Goat anti-rabbit HRP-conjugated IgG at 1/5000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

References

This product has been referenced in:
  • Watanabe N  et al. Requirement of Gaq/Ga11 Signaling in the Preservation of Mouse Intestinal Epithelial Homeostasis. Cell Mol Gastroenterol Hepatol 2:767-782.e6 (2016). Read more (PubMed: 28174748) »
  • Hughes S  et al. Using siRNA to define functional interactions between melanopsin and multiple G Protein partners. Cell Mol Life Sci 72:165-79 (2015). IHC . Read more (PubMed: 24958088) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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