Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab97501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 40 kDa).

Target

  • FunctionGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
  • Tissue specificityRetinal rod outer segment.
  • Involvement in diseaseDefects in GNAT2 are the cause of achromatopsia type 4 (ACHM4) [MIM:139340]. Achromatopsia is an autosomal recessively inherited visual disorder that is present from birth and that features the absence of color discrimination.
  • Sequence similaritiesBelongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACHM4 antibody
    • Cone type transducin alpha subunit antibody
    • GNAT 2 antibody
    • GNAT C antibody
    • Gnat2 antibody
    • GNAT2_HUMAN antibody
    • GNATC antibody
    • Guanine nucleotide binding protein (G protein) alpha transducing activity polypeptide 2 antibody
    • Guanine nucleotide binding protein G t subunit alpha 2 antibody
    • Guanine nucleotide-binding protein G(t) subunit alpha-2 antibody
    • Transducin alpha 2 antibody
    • Transducin alpha-2 chain antibody
    • Transducin alpha2 antibody
    • Transducin cone specific alpha polypeptide antibody
    see all

Anti-GNAT2 antibody images

  • All lanes : Anti-GNAT2 antibody (ab97501) at 1 µg/ml

    Lane 1 : Mouse Retina Tissue Lysate
    Lane 2 : Rat Retina Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

References for Anti-GNAT2 antibody (ab97501)

This product has been referenced in:
  • Datta P  et al. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proc Natl Acad Sci U S A 112:E4400-9 (2015). WB ; Mouse . Read more (PubMed: 26216965) »
  • Sato K  et al. M-opsin protein degradation is inhibited by MG-132 in Rpe65(-/-) retinal explant culture. Mol Vis 18:1516-25 (2012). WB ; Mouse . Read more (PubMed: 22736942) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Retinal)
Loading amount 10 µg
Specification Retinal
Gel Running Conditions Reduced Denaturing (10% Tris Glycine Gel)
Blocking step 1:1 PBS: Licor Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Username

Dr. Chris Tracy

Verified customer

Submitted Aug 24 2012

Thank you for calling Abcam earlier today,

Please find the immunogen sequence for ab97501 below:
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"