Overview

  • Product name
  • Description
    Rabbit polyclonal to GNAT2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GNAT2.

  • Positive control
    • This antibody gave a positive signal in both Rat and Mouse Retina tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab97501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 40 kDa).

Target

  • Function
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
  • Tissue specificity
    Retinal rod outer segment.
  • Involvement in disease
    Defects in GNAT2 are the cause of achromatopsia type 4 (ACHM4) [MIM:139340]. Achromatopsia is an autosomal recessively inherited visual disorder that is present from birth and that features the absence of color discrimination.
  • Sequence similarities
    Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACHM4 antibody
    • Cone type transducin alpha subunit antibody
    • GNAT 2 antibody
    • GNAT C antibody
    • Gnat2 antibody
    • GNAT2_HUMAN antibody
    • GNATC antibody
    • Guanine nucleotide binding protein (G protein) alpha transducing activity polypeptide 2 antibody
    • Guanine nucleotide binding protein G t subunit alpha 2 antibody
    • Guanine nucleotide-binding protein G(t) subunit alpha-2 antibody
    • Transducin alpha 2 antibody
    • Transducin alpha-2 chain antibody
    • Transducin alpha2 antibody
    • Transducin cone specific alpha polypeptide antibody
    see all

Anti-GNAT2 antibody images

  • All lanes : Anti-GNAT2 antibody (ab97501) at 1 µg/ml

    Lane 1 : Mouse Retina Tissue Lysate
    Lane 2 : Rat Retina Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

References for Anti-GNAT2 antibody (ab97501)

This product has been referenced in:
  • Datta P  et al. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proc Natl Acad Sci U S A 112:E4400-9 (2015). WB ; Mouse . Read more (PubMed: 26216965) »
  • Sato K  et al. M-opsin protein degradation is inhibited by MG-132 in Rpe65(-/-) retinal explant culture. Mol Vis 18:1516-25 (2012). WB ; Mouse . Read more (PubMed: 22736942) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Retinal)
Loading amount
10 µg
Specification
Retinal
Gel Running Conditions
Reduced Denaturing (10% Tris Glycine Gel)
Blocking step
1:1 PBS: Licor Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Username

Dr. Chris Tracy

Verified customer

Submitted Aug 24 2012

Thank you for calling Abcam earlier today,

Please find the immunogen sequence for ab97501 below:
********

If there is anything else I can help you with.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up