Overview

  • Product nameAnti-GNB1 antibody
    See all GNB1 primary antibodies
  • Description
    Rabbit polyclonal to GNB1
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Sheep, Cow, Human, Fruit fly (Drosophila melanogaster)
    Predicted to work with: Rat, Dog, Xenopus laevis, Zebrafish, Amphibians, Chinese Hamster
  • Immunogen

    Synthetic peptide corresponding to Human GNB1 aa 8-25.
    Sequence:

    RQEAEQLKNQIRDARKAC


    (Peptide available as ab4972)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notes Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to, and interpreted by, the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab3433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Can be blocked with GNB1 peptide (ab4972).

Target

Anti-GNB1 antibody images

  • Western blot of Tr beta on sheep retinal extracts using ab3433. Western blot of GNB1 on sheep retinal extracts using ab3433.

References for Anti-GNB1 antibody (ab3433)

ab3433 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"