Overview

  • Product nameAnti-GNB1L antibody
  • Description
    Rabbit polyclonal to GNB1L
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    A region within synthetic peptide: RVFHWRTMQP LAVLAFHSAA VQCVAFTADG LLAAGSKDQR ISLWSLYPRA, corresponding to amino acids 278-327 of Human GNB1L

  • Positive control
    • Jurkat cell lysate, human kidney and skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab50998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:12500

Target

  • Tissue specificityUbiquitous. Highly expressed in heart, liver, skeletal muscle, kidney, spleen, thymus and pancreas. Detected at low levels in lung, placenta and brain.
  • Sequence similaritiesContains 6 WD repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • DGCRK3 antibody
    • ESTM55 antibody
    • fb98e06 antibody
    • fj09d12 antibody
    • FKSG1 antibody
    • G protein subunit beta-like protein 1 antibody
    • G-protein beta subunit-like protein antibody
    • GNB1L antibody
    • GNB1L_HUMAN antibody
    • guanine nucleotide binding protein (G protein), beta polypeptide 1-like antibody
    • guanine nucleotide binding protein beta-subunit-like polypeptide antibody
    • Guanine nucleotide-binding protein subunit beta-like protein 1 antibody
    • GY2 antibody
    • KIAA1645 antibody
    • WD repeat-containing protein 14 antibody
    • WD40 repeat-containing protein deleted in VCFS antibody
    • WDR14 antibody
    • WDVCF antibody
    • wu:fb98e06 antibody
    • wu:fj09d12 antibody
    • zgc:55774 antibody
    see all

Anti-GNB1L antibody images

  • Anti-GNB1L antibody (ab50998) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • ab50998 at 4ug/ml staining human GNB1L in epithelial cells of renal tubule in human kidney tissue by Immunohistochemistry, Paraffin embedded tissue.ab50998 at 4ug/ml staining human GNB1L in epithelial cells of renal tubule in human kidney tissue by Immunohistochemistry, Paraffin embedded tissue.
  • ab50998 at 4ug/ml staining human GNB1L in squamous epithelial cells of human skin by Immunohistochemistry, Paraffin embedded tissue.ab50998 at 4ug/ml staining human GNB1L in squamous epithelial cells of human skin by Immunohistochemistry, Paraffin embedded tissue.
  • All lanes : Anti-GNB1L antibody (ab50998) at 2.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 35 kDa

References for Anti-GNB1L antibody (ab50998)

ab50998 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab50998.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"