Recombinant
RabMAb

Anti-GNB2 antibody [EPR3261Y] (ab81272)

Overview

  • Product name
    Anti-GNB2 antibody [EPR3261Y]
    See all GNB2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3261Y] to GNB2
  • Tested applications
    Suitable for: IHC-P, WB, ICC, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminal of human GNB2

  • Positive control
    • SHSY5Y, Jurkat and NIH3T3 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab81272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/5000 - 1/20000. Predicted molecular weight: 37 kDa.
ICC 1/100 - 1/250.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/20.

Target

  • Function
    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
  • Sequence similarities
    Belongs to the WD repeat G protein beta family.
    Contains 7 WD repeats.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • G protein beta 2 subunit antibody
    • G protein subunit beta 2 antibody
    • G protein subunit beta-2 antibody
    • GBB2_HUMAN antibody
    • Gnb2 antibody
    • Gnb2l1 antibody
    • Guanine nucleotide binding protein beta 2 subunit antibody
    • Guanine nucleotide binding protein G I G S G T beta 2 subunit 2 antibody
    • Guanine nucleotide binding protein G protein beta polypeptide 2 antibody
    • Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 antibody
    • OTTHUMP00000174601 antibody
    • OTTHUMP00000174602 antibody
    • RACK1 antibody
    • Receptor for activated C kinase antibody
    • Receptor of activated protein kinase C 1 antibody
    • Signal transducing guanine nucleotide binding regulatory protein beta antibody
    • Transducin beta chain 2 antibody
    see all

Images

  • All lanes : Anti-GNB2 antibody [EPR3261Y] (ab81272) at 1/20000 dilution

    Lane 1 : SHSY5Y cell lysates
    Lane 2 : Jurkat cell lysates
    Lane 3 : NIH3T3 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit HRP labelled IgG at 1/2000 dilution

    Predicted band size : 37 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References

ab81272 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12% acrylamide gel)
Sample
Human Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 13 2014

Merci de nous avoir contactés.

Je suis désolé d'apprendre que vous rencontrez des difficultés à obtenir de bons résultats avec ab81272 en Western Blot.

Notre politique est d'offrir tout d'a...

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Je suis désolée d'apprendre que ab81272 ne vous donne pas de bons résultats en Western blot. Je vais regarder quel autre anticorps pourrait convenir mieux. Pour ceci j'aura par contre besoin de savoir aussi contre quel espèce l'anticorps doit être diri...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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