The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionGuanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Sequence similaritiesBelongs to the WD repeat G protein beta family. Contains 7 WD repeats.
ab109466 (3.8µg/ml) staining of paraffin embedded Human Cerebellum shows strong axonal staining in the Molecular layer and less so in the Granular layer. Tissue treated: Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
References for Anti-GNB3 antibody (ab109466)
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