Recombinant
RabMAb

Anti-GNB3 antibody [EPR8862] (ab154866)

Overview

  • Product name
    Anti-GNB3 antibody [EPR8862]
    See all GNB3 primary antibodies
  • Description
    Rabbit monoclonal [EPR8862] to GNB3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues within Human GNB3 (Uniprot ID P16520).

  • Positive control
    • HepG2, A673 cell lysate; Human fetal brain tissue lysate; Human fetal lung tissue lysate; Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab154866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
    • Sequence similarities
      Belongs to the WD repeat G protein beta family.
      Contains 7 WD repeats.
    • Information by UniProt
    • Database links
    • Alternative names
      • G protein beta 3 subunit antibody
      • GBB3_HUMAN antibody
      • GNB3 antibody
      • GNB3 guanine nucleotide binding protein (G protein), beta polypeptide 3 antibody
      • GTP binding regulatory protein beta 3 chain antibody
      • Guanine nucleotide binding protein (G protein) beta polypeptide 3 antibody
      • Guanine nucleotide binding protein G antibody
      • Guanine nucleotide binding protein G(I)/G(S)/G(T) beta subunit 3 antibody
      • Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 antibody
      • Hypertension associated protein antibody
      • IG antibody
      • SG antibody
      • Transducin beta chain 3 antibody
      • Tsubunit beta 3 antibody
      see all

    Images

    • All lanes : Anti-GNB3 antibody [EPR8862] (ab154866) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Human fetal brain tissue lysate
      Lane 3 : A673 cell lysate
      Lane 4 : Human fetal lung tissue lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 37 kDa
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GNB3 with ab154866 at 1/50.
    • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154866 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154866 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal heart tissue using ab154866 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154866 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human retina tissue using ab154866 showing +ve staining.

    References

    ab154866 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As



    The immunogen used to raise the antibody Anti-GNB3 antibody [EPR8862] (ab154866) is a 14 amino acid residue synthetic peptide taken from within the region 100-150 of Human GNB3 (Uniprot ID P16520).

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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