Anti-GNMT antibody (ab118298)

Overview

  • Product nameAnti-GNMT antibody
    See all GNMT primary antibodies
  • Description
    Rabbit polyclonal to GNMT
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • Immunogen

    Synthetic peptide corresponding toa region within N terminal amino acids 12-61 (GVAAEGLPDQ YADGEAARVW QLYIGDTRSR TAEYKAWLLG LLRQHGCHRV) of Mouse GNMT (NP_034451).

  • Positive control
    • Mouse small intestine lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab118298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionCatalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.
  • Tissue specificityAbundant in liver.
  • Involvement in diseaseDefects in GNMT are the cause of glycine N-methyltransferase deficiency (GNMT deficiency) [MIM:606664]; also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases.
  • Sequence similaritiesBelongs to the glycine N-methyltransferase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.1.1.20 antibody
    • Glycine N methyltransferase antibody
    • Glycine N-methyltransferase antibody
    • Gnmt antibody
    • GNMT_HUMAN antibody
    • OTTHUMP00000016412 antibody
    see all

Anti-GNMT antibody images

  • Anti-GNMT antibody (ab118298) at 1 µg/ml + Mouse small intestine lysate at 10 µg

    Predicted band size : 33 kDa

    Gel concentration: 12%

     

    Secondary antibody - anti-rabbit HRP (ab6721)

References for Anti-GNMT antibody (ab118298)

ab118298 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Liver)
Loading amount 10 µg
Specification Liver
Gel Running Conditions Reduced Non-Denaturing (Native) (10% Acrylamide resolving gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Feb 07 2013

DISCOUNT CODE: XXXXXXX
Expiration date: 17-01-2013
Value: £235

I am very pleased to hear you would like to accept our offer and test ab118298 in Rat. This code will give you ab118298 off your next order before the expiration date...

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The immunogen of antibody ab118298 is 98% similar with rat; though we have not tested the antibody with Rat samples however it is most likely to cross react.

http://www.abcam.com/gnmt-antibody-ab118298.ht...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"