Overview

  • Product name
  • Description
    Rabbit polyclonal to GNPAT
  • Specificity
    ab75060 detects endogenous levels of total GNPAT protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from C-terminal region of human GNPAT.

  • Positive control
    • Extracts from HT-29 cells and RAW264.7cells

Properties

Applications

Our Abpromise guarantee covers the use of ab75060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 77 kDa.
ELISA 1/20000.

Target

  • Pathway
    Membrane lipid metabolism; glycerophospholipid metabolism.
  • Involvement in disease
    Defects in GNPAT are the cause of rhizomelic chondrodysplasia punctata type 2 (RCDP2) [MIM:222765]. RDCP2 is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation.
  • Sequence similarities
    Belongs to the GPAT/DAPAT family.
  • Domain
    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localization
    Peroxisome membrane. Exclusively localized to the lumenal side of the peroxisomal membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acyl CoA dihydroxyacetonephosphateacyltransferase antibody
    • Acyl-CoA:dihydroxyacetonephosphateacyltransferase antibody
    • DAP AT antibody
    • DAP-AT antibody
    • DAPAT antibody
    • DHAP AT antibody
    • DHAP-AT antibody
    • DHAPAT antibody
    • Dihydroxyacetone phosphate acyltransferase antibody
    • EC 2.3.1.42 antibody
    • Glycerone-phosphate O-acyltransferase antibody
    • Glyceronephosphate O acyltransferase antibody
    • GNPAT antibody
    • GNPAT_HUMAN antibody
    • OTTHUMP00000036147 antibody
    see all

Images

  • All lanes : Anti-GNPAT antibody (ab75060) at 1/500 dilution

    Lane 1 : extracts from HT-29 cells
    Lane 2 : extracts from RAW264.7cells
    Lane 3 : extracts from HT-29 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Hossain MS  et al. Neuronal Orphan G-Protein Coupled Receptor Proteins Mediate Plasmalogens-Induced Activation of ERK and Akt Signaling. PLoS One 11:e0150846 (2016). WB . Read more (PubMed: 26934370) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Loading amount
10 µg
Specification
C2C12
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Nov 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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