Overview

  • Product name
  • Description
    Mouse polyclonal to GNPTG
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human GNPTG protein.

  • Positive control
    • GNPTG transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at an assay dependent dilution. Detects a band of approximately 28 kDa (predicted molecular weight: 32 kDa).
    Detection Pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      May recognize the substrate of GlcNAc-1-phosphotransferase but also the lysosomal proteins with mannose-6-phosphate residues.
    • Cellular localization
      Golgi Apparatus and Secreted
    • Database links
    • Alternative names
      • GlcNAc phosphotransferase gamma subunit antibody
      • GlcNAc-1-phosphotransferase subunit gamma antibody
      • GNPTAG antibody
      • LP2537 antibody
      • N-acetylglucosamine-1-phosphate transferase gamma subunit antibody
      • RJD9 antibody
      • UDP-N-acetylglucosamine-1-phosphotransferase subunit gamma antibody
      see all

    Images

    • All lanes : Anti-GNPTG antibody (ab67775) at 1/500 dilution

      Lane 1 : GNPTG transfected 293T cell lysate
      Lane 2 : Non transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 32 kDa
      Observed band size : 28 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Beretta GL  et al. Increased levels and defective glycosylation of MRPs in ovarian carcinoma cells resistant to oxaliplatin. Biochem Pharmacol 79:1108-17 (2010). Read more (PubMed: 20005867) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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