Overview

  • Product name
  • Description
    Rabbit polyclonal to GnRH
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IHC-FoFr, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Hamster, Pig, Xenopus laevis, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse GnRH aa 22-31. Modified residues - 22 with pyrrolidone carboxylic acid, 31 with glycine amide.
    Sequence:

    EHWSYGLRPG


    (Peptide available as ab41769)

  • Positive control
    • Mouse brain tissue sections.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purification notes
    Purified via ammonium sulfate precipitation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/5000.

4% paraformaldehyde is recommended as a fixative.

ICC/IF 1/1000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22348098
IHC-Fr Use at an assay dependent concentration. PubMed: 19479999

Target

  • Relevance
    Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle. Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • GnRH associated peptide 1 antibody
    • GNRH1 antibody
    • Gonadotrophin releasing hormone 1 antibody
    • GRH antibody
    • HH12 antibody
    • LHRH antibody
    • LNRH antibody
    • Luliberin I antibody
    • Luteinizing releasing hormone antibody
    • Lutenizing hormome releasing hormone antibody
    • Progonadoliberin 1 antibody
    • Progonadoliberin I antibody
    • Prolactin release inhibiting factor antibody
    see all

Images

  • Immunolocalization of GnRH in mouse embryo forebrain using ab5617 at a 1/1000 dilution.

References

This product has been referenced in:
  • Soylu-Kucharz R  et al. Metabolic and behavioral effects of mutant huntingtin deletion in Sim1 neurons in the BACHD mouse model of Huntington's disease. Sci Rep 6:28322 (2016). IHC . Read more (PubMed: 27334347) »
  • Song B  et al. Effect of Aqueous Extract from Morinda officinalis F. C. How on Microwave-Induced Hypothalamic-Pituitary-Testis Axis Impairment in Male Sprague-Dawley Rats. Evid Based Complement Alternat Med 2015:360730 (2015). Read more (PubMed: 26435724) »

See all 10 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Cell (Hypothalamus)
Permeabilization
Yes - 0.5% Triton X-100
Specification
Hypothalamus
Blocking step
Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Kevin Zhang

Verified customer

Submitted Nov 17 2017

Application
IHC - Wholemount
Sample
Astatotilapia burtoni Tissue (Brain (CLARITY cleared with Passive clearing))
Specification
Brain (CLARITY cleared with Passive clearing)
Username

Sebastian Alvarado

Verified customer

Submitted May 02 2016

Thank you for contacting us. Both the ab5617 and ab16216 are raised against an immunogen synthetic peptide of HWSYGLRPG corresponding to amino acids 22-31 of GnRH. Comparing this to the corresponding sequence of Chicken GnRH (Swiss-Prot number ...

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Thank you for your enquiry. The concentration of the lot that received in November is approximately 1 mg/ml. Our current batch also has a concentration of approximately 1 mg/ml. Please contact us again if you have any additional questions.

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you dec...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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