• Product nameAnti-GnRH antibody
    See all GnRH primary antibodies
  • Description
    Rabbit polyclonal to GnRH
  • Tested applicationsSuitable for: ELISA, IHC-Fr, IHC-P, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human) conjugated to BSA.

  • Positive control
    • Hypothalamus
  • General notesLHRH is also known as Gonadotropin-releasing hormone (GNRH).



Our Abpromise guarantee covers the use of ab8491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesThis antibody is suitable for use on paraffin embedded tissues without enzymatic antigen retrieval
    Advised working dilutions are 1:50-1:100.
    Dilution buffer is 0.15 M PBS with 1% Ovalbumin and 0.09% sodium azide.
    Optimal dilution should be tested by serial dilution.
  • Target

    • RelevanceGonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle. Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • GnRH associated peptide 1 antibody
      • GNRH1 antibody
      • Gonadotrophin releasing hormone 1 antibody
      • GRH antibody
      • HH12 antibody
      • LHRH antibody
      • LNRH antibody
      • Luliberin I antibody
      • Luteinizing releasing hormone antibody
      • Lutenizing hormome releasing hormone antibody
      • Progonadoliberin 1 antibody
      • Progonadoliberin I antibody
      • Prolactin release inhibiting factor antibody
      see all

    References for Anti-GnRH antibody (ab8491)

    ab8491 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry and your detailed protocol. We are very sorry to hear that you are having problem with this antibody. We have sold several vials of this antibody and do not have any problem with it. According to the datasheet, this pr...

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    Unfortunately the epitope is not known. We know that it recognizes several epitopes on LH-RH. In the following reference there is data about the preparation of the antigen conjugate. Van Rees, et al., 1984, Acta Endocrinologica 104: 272-278

    I have been unable to obtain a protocol for the use of this antibody in IHC from the originator. Furthermore there are no published references, so all the information we have is on the datasheet where we advise that use in IHC on PES should be without ...

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