Overview

  • Product name
    Anti-GnRHR antibody [A9E4]
    See all GnRHR primary antibodies
  • Description
    Mouse monoclonal [A9E4] to GnRHR
  • Specificity
    It is unknown if ab24095 reacts with the receptor form 1 or 2.
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Pig
  • Immunogen

    Synthetic peptide:

    MANSASPEQNQHCSAINNSIPLMQGNLPY

    conjugated to BSA, corresponding to N terminal amino acids 1-29 of Human GnRHR

  • Positive control
    • IHC-Fr: human anterior pituitary sections WB/FACS: GT7-1 cells (hypothalamus cell line)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    A9E4
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/20 - 1/40.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA 1/100.
WB 1/20 - 1/40. Predicted molecular weight: 38 kDa.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • GnRH receptor antibody
    • GnRH-R antibody
    • GNRHR antibody
    • GNRHR_HUMAN antibody
    • GNRHR1 antibody
    • Gonadotropin releasing hormone receptor antibody
    • gonadotropin-releasing hormone (type 1) receptor 1 antibody
    • Gonadotropin-releasing hormone receptor antibody
    • GRHR antibody
    • HH7 antibody
    • leutinizing-releasing hormone receptor antibody
    • lh-rh antibody
    • LHRHR antibody
    • LRHR antibody
    • luliberin receptor antibody
    • luteinizing hormone releasing hormone receptor antibody
    • type I GnRH receptor antibody
    see all

Anti-GnRHR antibody [A9E4] images

  • Overlay histogram showing MCF7 cells stained with ab24095 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24095, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-GnRHR antibody [A9E4] (ab24095)

This product has been referenced in:
  • Seitz S  et al. Triple negative breast cancers express receptors for LHRH and are potential therapeutic targets for cytotoxic LHRH-analogs, AEZS 108 and AEZS 125. BMC Cancer 14:847 (2014). IHC ; Human . Read more (PubMed: 25410881) »

See 1 Publication for this product

Product Wall

Usually if you are trying to replicate our results, we first suggest trying the specific protocol listed on our datasheet.

For this antibody, we tested it in our lab under the conditions stated on the datasheet and this gave good results in o...

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Vielen Dank, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen und ein Bild mitzuschicken. Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten. Um Ihr Problem zu lösen, möchte ich die folgenden Vorschläge zu Ver...

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Thank you for your enquiry. The molecular weight that we have stated on our datasheet is the predicted molecular weight, base on Swiss Prot database. The actual molecular weight could be higher or lower and depends on factors like different modifica...

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Thank you for your enquiry. The immunogen sequence used to generate the antibody ab24095 is MANSASPEQNQHCSAINNSIPLMQGNLPY and a BLAST search showed 96% homology with both the isoforms 1 and 2, therefore we do not know if ab24095 reacts with receptor ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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