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Mouse polyclonal to GnRHR
Vector coding for a partial recombinant fusion protein, corresponding to amino acids 320-368 of Fruit fly (Drosophila melanogaster) GnRHR.
Target sequence used to make the antibody: GLYNIRGRMN NNNPSVNNRH TSLSNRLDSS NQLMQKQLTN NSLLNGRGQ.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 50% Glycerol, Whole serum
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 50 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Western blot - GnRHR antibody (ab52673)
All lanes : Anti-GnRHR antibody (ab52673) at 1/1000 dilution Lane 1 : total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen Lane 2 : total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein). Secondary Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution Predicted band size : 50 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"