Overview

  • Product name
  • Description
    Goat polyclonal to GnRHR
  • Specificity
    This antibody is expected to recognise both reported isoforms (NP_000397.1; NP_001012781.1).
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-QKWTQKKEKGK

    , corresponding to internal sequence amino acids 61-71 of Human GnRHR Peptide available as ab94807.

  • Positive control
    • Human Peripheral Blood Mononucleocyte lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/32000.

Peptide ELISA.

WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human GnRHR peptide (ab94807).

The observed molecular weight corresponds to earlier findings with products from other commercial sources.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • GnRH receptor antibody
    • GnRH-R antibody
    • GNRHR antibody
    • GNRHR_HUMAN antibody
    • GNRHR1 antibody
    • Gonadotropin releasing hormone receptor antibody
    • gonadotropin-releasing hormone (type 1) receptor 1 antibody
    • Gonadotropin-releasing hormone receptor antibody
    • GRHR antibody
    • HH7 antibody
    • leutinizing-releasing hormone receptor antibody
    • lh-rh antibody
    • LHRHR antibody
    • LRHR antibody
    • luliberin receptor antibody
    • luteinizing hormone releasing hormone receptor antibody
    • type I GnRH receptor antibody
    see all

Anti-GnRHR antibody images

  • Anti-GnRHR antibody (ab58561) at 0.5 µg/ml + Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer)

    Predicted band size : 38 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.

References for Anti-GnRHR antibody (ab58561)

This product has been referenced in:
  • Seitz S  et al. Triple negative breast cancers express receptors for LHRH and are potential therapeutic targets for cytotoxic LHRH-analogs, AEZS 108 and AEZS 125. BMC Cancer 14:847 (2014). ICC/IF ; Human . Read more (PubMed: 25410881) »

See 1 Publication for this product

Product Wall

We do not have data for reactivity with purified protein but I was able to align the immunogen amino acid sequence for this antibody, QKWTQKKEKGK, with the sequences you are interested in, to get a prediction of reactivity. In brief, the immunogen shar...

Read More

Thank you for contacting us.


I did a blast search and found that the immunogen sequence for ab58561 has only 82% homology with the mouse sequence. Base on sequence homology, the chance of cross reacting with mouse is not high. At the mo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up