Goat Anti-Armenian Hamster IgG H&L (Alkaline Phosphatase) (ab5746)

Overview

  • Product nameGoat Anti-Armenian Hamster IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Target speciesArmenian Hamster
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Armenian Hamster and Armenian Hamster Serum.
  • Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details
  • Immunogen

    Armenian Hamster IgG whole molecule

  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 0.05M Tris HCl, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose.
  • Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
Dot blot Use at an assay dependent dilution.
WB 1/500 - 1/2500.
ELISA 1/2000 - 1/25000.
IHC Use at an assay dependent dilution.

References for Goat Anti-Armenian Hamster IgG H&L (Alkaline Phosphatase) (ab5746)

ab5746 has not yet been referenced specifically in any publications.

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This secondary antibody can be used for IHC, and the staining detected using the NBT/BCIP substrate ab7468. Although we do not have an image using this specific secondary on file, I have attached an image of AP conjuga...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"