Overview

  • Product name
    Goat Anti-Chicken IgY H&L (6nm Gold)
    See all IgY secondary antibodies
  • Target species
    Chicken
  • Tested applications
    Suitable for: Electron Microscopymore details
  • Immunogen

    Chicken Ig whole molecule.

  • Conjugation
    Gold 6nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 6nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6nm particle size conjugate is less than 12 %.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution. The method of production of this product allows the small particle size of 6nm to be used instead of 5nm with the same sensitivity but increased visibility in the standard transmission electron microscope (See also van Bergen en Henegouwen & Leunissen, Histochemistry, 85, (1986), 81.)

    Light Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    This product has been referenced in:
    • Stanton RJ  et al. Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication. J Clin Invest 120:3191-208 (2010). Electron Microscopy . Read more (PubMed: 20679731) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for your reply. I am sorry we missed eachother yesterday.


    I'm pleased to help answer your questions:

    1. Icanconfirm weregrettablydo not currently have a goat anti-mouse IgG (H+L) gold conjugated and pre-adsorbed in ...

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    Thank you once again for your recent enquiries.

    Following on from my previous email, I can now confirm that ab39604 Goat polyclonal Secondary Antibody to Chicken IgYis cross adsorbed against human, mouse, rabbit, rat and sheep serum proteins...

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    Thankyou for your telephone call yesterday afternoon. I am sorry to hear you have some concerns regardingthe results in TEM from ab105265.

    I would like to reassure you that this antibody is tested and covered by our guarantee forEM and human...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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