Overview

  • Product name
    Goat Anti-Chicken IgY H&L
    See all IgY secondary antibodies
  • Host species
    Goat
  • Target species
    Chicken
  • Specificity
    The antibody reacts with chicken immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Human IgG, mouse IgG, rat IgG and rabbit IgG, less than 2%.
  • Tested applications
    Suitable for: ELISA, IHC-P, WB, IPmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/200000.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Images

  • Cross-reactivity of the polyclonal secondary antibody ab182019 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1µg/ml (50µl/well) amd incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182019 was then added starting at 1 µg/ml and gradually diluted 1:4 (50µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1:20,000 dilution (50µl/well), followed by incubation for 1h at RT.

    For the batch tested, ab182019 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG and rabbit IgG.

References

ab182019 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab182019.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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