Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

Overview

  • Product nameGoat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed
    See all IgY secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alexa Fluor® 594), pre-adsorbed
  • Target speciesChicken
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • ConjugationAlexa Fluor® 594. Ex: 590nm, Em: 617nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/2000.

Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed images

  • ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 10µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150176 Alexa Fluor® 594 goat anti-chicken IgY (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150176 Alexa Fluor® 594 goat anti-chicken IgY (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

ab150176 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Immunocytochemistry
Application:Immunocytochemistry
Sample: Species: Human
Blocking step:1% BSAin 1xTBS
Primary Antibody: Anti-200 kD Neurofilament Heavy antibody (ab4680), 2 hours Temperature: 4°C, Diluent: 1xTBS with 0.1% BSA
Secondary Antibody: ab150176, Dilution: 1/1000 Incubation time:1 hours at RT Diluent: 1xTBS with 0.1% BSA
Detection method: 594 excitation
Username

Abcam user community

Verified customer

Submitted Feb 18 2016

Application Immunocytochemistry
Application: ICC
Sample: Species: GFP plasmid overexpression
Blocking step:1X BSA
Primary Antibody: anti-GFP (ab13970), Dilution: 1/200 Incubation time:13 hours at 4°C Diluent: 1xTBS
Secondary Antibody: ab150176, Dilution: 1/400 Incubation time:2 hours at RT Diluent: 1xTBS
Detection method: 594 excitation
Username

Abcam user community

Verified customer

Submitted Nov 02 2015

Abreviews
Application Western blot
Application: Western blot

Sample: Species: Mouse Type: Cell lysate - whole cell

Loading amount: 25 µg, Specification: Cardiomyocytes overexpressed with GFP reporter plasmid

Gel Running Conditions: Reduced Denaturing (15% PAGE)

Blocking step: Milk as blocking agent for 1 hour at RT°C Blocking Concentration: 5%


Primary Antibody: anti-GFP (ab13970), Dilution: 1/2000 Incubation time:4 hours at 4°C Diluent: 1xTBS


Secondary Antibody: ab150176, Dilution: 1/5000 Incubation time:2 hours at RT Diluent: 1xTBS


Detection method: 594 excitation


Bands: Specific: ~25 kDa, Non-specific: none
Username

Abcam user community

Verified customer

Submitted Nov 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"