Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed (ab97142)

Overview

  • Product name
    Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed
    See all IgY secondary antibodies
  • Target species
    Chicken
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/2500 - 1/25000. (Primary ELISA)
WB 1/2500 - 1/25000. (Chemiluminescent)

References for Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed (ab97142)

This product has been referenced in:
  • Lin CY  et al. Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion. Biotechnol Biofuels 9:225 (2016). WB, SDS-PAGE . Read more (PubMed: 27777626) »

See 1 Publication for this product

Product Wall

Vielen Dank für Ihre Anfrage.

Ich habe Ihnen hier vier Antikörper herausgesucht, die wenig bzw. gar keine Kreuzreaktion mit dem Maus-Protein aufweisen, so dass Sie nur das humane Protein im Mausgewebe detektieren sollten.

Drei der...

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Thank you for contacting us. Ab97141 is an un-conjugated secondary antibody to Chicken IgY. In order of using this antibody in different applications, you would need a fluorophore conjugated anti goat antibody. However it will depend on the app...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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