• Product nameGoat Anti-Chicken IgY H&L (Biotin)
    See all IgY secondary antibodies
  • Target speciesChicken
  • Tested applicationsSuitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    chicken IgY whole molecule

  • ConjugationBiotin


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityIgY fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule
  • ClonalityPolyclonal
  • IsotypeIgY
  • Research areas


Our Abpromise guarantee covers the use of ab6876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/3000.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/3000 - 1/30000.

Goat Anti-Chicken IgY H&L (Biotin) images

  • ab89999 staining GFAP in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 22°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in  TBS/BSA/Azide) for 2 hours at 22°C. A biotin-conjugated goat anti-chicken IgY H&L (ab6876) (1/100) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analyis of Human lung tissue stained with a chicken primary antibody, followed by ab6876 at 1/500 dilution and horseradish peroxidase-conjugated streptavidin. Staining was detected using DAB.

References for Goat Anti-Chicken IgY H&L (Biotin) (ab6876)

This product has been referenced in:
  • Huang B  et al. Dysregulation of Notch and ERa signaling in AhR-/- male mice. Proc Natl Acad Sci U S A 113:11883-11888 (2016). IHC . Read more (PubMed: 27688768) »
  • Huang B  et al. Differential expression of estrogen receptor a, ß1, and ß2 in lobular and ductal breast cancer. Proc Natl Acad Sci U S A 111:1933-8 (2014). Read more (PubMed: 24449868) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed paraffin wax section was subjected to HIER before incubating in anti GFP ( ab13970). Section was then incubated with ab6876 ( 1/200) followed by stABCpx. GFP was visualised using DAB chromogen

Mr. Carl Hobbs

Verified customer

Submitted Sep 25 2013

I have been informed that amine reactive biotins were used to conjugate Lysine sites of antibodies. It is not only in the Fc region. Please do not hesitate to contact us again if you need more information,

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. At this time, we do not have any data about cross-reactivity with human, rabbit and mouse proteins.

We don't have any data on the sensitivity of antibodies from DAKO, so its' difficult to compare. We do know that this antibody recognises 1µg chicken IgG at a working dilution of at least 1/20000.