Overview

  • Product name
    Goat Anti-Chicken IgY H&L (Biotin)
    See all IgY secondary antibodies
  • Target species
    Chicken
  • Tested applications
    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    chicken IgY whole molecule

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgY fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/3000.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/3000 - 1/30000.

Images

  • ab89999 staining GFAP in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 22°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in  TBS/BSA/Azide) for 2 hours at 22°C. A biotin-conjugated goat anti-chicken IgY H&L (ab6876) (1/100) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analyis of Human lung tissue stained with a chicken primary antibody, followed by ab6876 at 1/500 dilution and horseradish peroxidase-conjugated streptavidin. Staining was detected using DAB.

References

This product has been referenced in:
  • Huang B  et al. Dysregulation of Notch and ERa signaling in AhR-/- male mice. Proc Natl Acad Sci U S A 113:11883-11888 (2016). IHC . Read more (PubMed: 27688768) »
  • Huang B  et al. Differential expression of estrogen receptor a, ß1, and ß2 in lobular and ductal breast cancer. Proc Natl Acad Sci U S A 111:1933-8 (2014). Read more (PubMed: 24449868) »

See all 4 Publications for this product

Customer reviews and Q&As

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Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed paraffin wax section was subjected to HIER before incubating in anti GFP ( ab13970). Section was then incubated with ab6876 ( 1/200) followed by stABCpx. GFP was visualised using DAB chromogen
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Mr. Carl Hobbs

Verified customer

Submitted Sep 25 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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