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Product name Goat Anti-Chicken IgY H&L (Biotin) See all IgY secondary antibodies
Target species Chicken
Specificity By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
Tested applications ELISA, IHC-P, ICC/IF, WB more details
Conjugation Biotin Properties
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
Purity Immunogen affinity purified
Purification notes This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
Conjugation notes Biotin/ antibody protein ratio is 4:1
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20000 - 1/400000. (Primary).
1/500 - 1/5000.
1/200 - 1/1000.
1/20000 - 1/400000. (Chemiluminescent).
Goat Anti-Chicken IgY H&L (Biotin) images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab97133)
Ab97133 was used at dilution 1/200 with the primary antibody ab81755 in IHC-P. See the review on ab81755.
References for Goat Anti-Chicken IgY H&L (Biotin) (ab97133)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"