By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
Ab97133 was used at dilution 1/200 with the primary antibody ab81755 in IHC-P. See the review on ab81755.
References for Goat Anti-Chicken IgY H&L (Biotin) (ab97133)
This product has been referenced in:
Pasini FS et al. A gene expression profile related to immune dampening in the tumor microenvironment is associated with poor prognosis in gastric adenocarcinoma. J Gastroenterol49:1453-66 (2014).
Read more (PubMed: 24217965) »