Goat Anti-Chicken IgY H&L (Cy3 ®) preadsorbed (ab97145)

Overview

  • Product nameGoat Anti-Chicken IgY H&L (Cy3 ®) preadsorbed
    See all IgY secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Cy3 ®), pre-adsorbed
  • Target speciesChicken
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • Immunogen

    Other Immunogen Type corresponding to Chicken IgY.

  • ConjugationCy3 ®. Ex: 552nm, Em: 565nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/100 - 1/1000.

References for Goat Anti-Chicken IgY H&L (Cy3 ®) preadsorbed (ab97145)

This product has been referenced in:

See 1 Publication for this product

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As we discussed, please send me the images of the staining. I would suggest using a positive control and confirming the GFP transfection, but trying a fluorophore from an infrared channel where the auto-fluorescence is lower may be a good sol...

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I have included with this email 2 documents about autofluorescence and tips for reducing this as requested.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"