Directly conjugated antibodies for immunofluorescence

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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab46969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-Fr
  • Application notesICC/IF: 1/250.
    IHC-Fr: Use at an assay dependent dilution (PMID 18583367).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat Anti-Chicken IgY H&L (FITC) images

    • ab4674 staining GFAP in human brain cells by Flow Cytometry. Cells were cultured in stem cell media RHB-A and collected using 0.05% Trypsin. The sample was incubated with the primary antibody (1/100 in PBS + 10% FCS) for 20 minutes at 25°C. A FITC-conjugated Goat anti-chicken IgY (ab46969) (1/100) was used as the secondary antibody.

       

      See Abreview

    References for Goat Anti-Chicken IgY H&L (FITC) (ab46969)

    This product has been referenced in:
    • Serra C  et al. Testosterone Improves the Regeneration of Old and Young Mouse Skeletal Muscle. J Gerontol A Biol Sci Med Sci : (2012). Read more (PubMed: 22499765) »
    • Anesti AM  et al. Efficient delivery of RNA Interference to peripheral neurons in vivo using herpes simplex virus. Nucleic Acids Res 36:e86 (2008). IHC-Fr . Read more (PubMed: 18583367) »

    See all 2 Publications for this product

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    DISCOUNT CODE for ab4674 Anti-GFAP antibody - Astrocyte Marker: XXXXX
    DISCOUNT CODE for ab46969 Goat anti-Chicken IgY H&L (FITC) secondary antibody: YYYYYYY


    Expiration date: ZZZZZZZ

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"