Our Abpromise guarantee covers the use of ab46969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab4674 staining GFAP in human brain cells by Flow Cytometry. Cells were cultured in stem cell media RHB-A and collected using 0.05% Trypsin. The sample was incubated with the primary antibody (1/100 in PBS + 10% FCS) for 20 minutes at 25°C. A FITC-conjugated Goat anti-chicken IgY (ab46969) (1/100) was used as the secondary antibody.