Overview

  • Product nameGoat Anti-Chicken IgY H&L (HRP)
    See all IgY secondary antibodies
  • Target speciesChicken
  • Tested applicationsICC/IF, Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    chicken IgY whole molecule

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate, 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • ClonalityPolyclonal
  • IsotypeIgY
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/10000.
Dot Blot Use at an assay dependent dilution.
ELISA 1/50000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB 1/2000 - 1/20000. PubMed: 16543490

Goat Anti-Chicken IgY H&L (HRP) images

  • IHC image of beta Actin staining in Human normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab13822, 10µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6877, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab6877 was used at dilution 1/1000 with the primary antibody ab25912 in WB. See the review on ab25912.
  • Ab6877 was used at dilution 1/200 with the primary antibody ab17517 in IHC-P. See the review on ab17517.

References for Goat Anti-Chicken IgY H&L (HRP) (ab6877)

This product has been referenced in:
  • Jensen SA  et al. C-terminal propeptide is required for fibrillin-1 secretion and blocks premature assembly through linkage to domains cbEGF41-43. Proc Natl Acad Sci U S A 111:10155-60 (2014). Read more (PubMed: 24982166) »
  • de Groot RE  et al. Protein kinase CK2 is required for Wntless internalization and Wnt secretion. Cell Signal 26:2601-2605 (2014). Read more (PubMed: 25178265) »

See all 12 Publications for this product

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"