• Product name
    Goat Anti-Chicken IgY H&L (HRP)
    See all IgY secondary antibodies
  • Target species
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgY and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes
    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality
  • Isotype
  • General notes
    Part of the AbExcel range.
  • Research areas


Our Abpromise guarantee covers the use of ab97135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

Goat Anti-Chicken IgY H&L (HRP) images

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 seconds

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 90 seconds
  • Ab97135 used to detect a chicken anti-beta Galactosidase antibody (ab9361). Ab97135 used at a concentration of 1:2000 in 5% milk blocking buffer in 0.1% Tween, 1x PBS. Whole lysates from DH5a (E.coli). Specific band at approx. 110 KDa. Non-specific bands: 70 Kda. NB. Gels run on 10% Acrylamide gels.

    This image is courtesy of an anonymous Abreview.

References for Goat Anti-Chicken IgY H&L (HRP) (ab97135)

This product has been referenced in:
  • Wolf L  et al. Receptor for advanced glycation endproducts (RAGE) maintains pulmonary structure and regulates the response to cigarette smoke. PLoS One 12:e0180092 (2017). WB ; Mouse . Read more (PubMed: 28678851) »
  • Amara S  et al. Critical role of SIK3 in mediating high salt and IL-17 synergy leading to breast cancer cell proliferation. PLoS One 12:e0180097 (2017). Read more (PubMed: 28658303) »

See all 9 Publications for this product

Product Wall

Western blot
Antibody was diluted 1/2000 in TBST and incubated 1h at RT
Detection performed using ECL+
Primary antibody used was ab13989.

Abcam user community

Verified customer

Submitted Oct 01 2015

Hello! The reply from the lab is below, please let me know if you have any further questions!

Two responses: The first is that the client should NOT use PVDF or any other nylon-based membranes. Chicken antibodies bind non-specifica...

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Vielen Dank für Ihre Antwort.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie besprochen, habe ich eine kostenlose Ersatzlieferung des ab97135 für Sie in Auftrag gegeben. Sie hat die Referenznummer 1212...

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Vielen dank für Ihre Antwort.

In diesem Fall würde ich Ihnen gerne anbieten nach dem Ausschlussverfahren vorzugehen, wenn Sie damit einverstanden sind.

Ich würde Ihnen gerne ein kostenloses Ersatzprodukt für den se...

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Vielen Dank für die Email und die Zusendung des ausgefüllten Fragebogens. Anja ist leider außer Haus. Wenn es Ihnen recht ist würde ich mich gerne in dieser Zeit um Ihren Fall kümmern.

Es tut mir Leid, dass sie Proble...

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