Overview

  • Product nameGoat Anti-Chicken IgY H&L (HRP)
    See all IgY secondary antibodies
  • Target speciesChicken
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsICC, IHC-P, ELISA, WBmore details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

Goat Anti-Chicken IgY H&L (HRP) images


  • developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 seconds

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 90 seconds
  • Ab97135 used to detect a chicken anti-beta Galactosidase antibody (ab9361). Ab97135 used at a concentration of 1:2000 in 5% milk blocking buffer in 0.1% Tween, 1x PBS. Whole lysates from DH5a (E.coli). Specific band at approx. 110 KDa. Non-specific bands: 70 Kda. NB. Gels run on 10% Acrylamide gels.

    This image is courtesy of an anonymous Abreview.

References for Goat Anti-Chicken IgY H&L (HRP) (ab97135)

This product has been referenced in:
  • Wang C  et al. The role of pro-inflammatory S100A9 in Alzheimer's disease amyloid-neuroinflammatory cascade. Acta Neuropathol 127:507-22 (2014). Read more (PubMed: 24240735) »
  • Cachón-González MB  et al. Reversibility of neuropathology in Tay-Sachs-related diseases. Hum Mol Genet N/A:N/A (2013). WB . Read more (PubMed: 24057669) »

See all 2 Publications for this product

Product Wall

Hello! The reply from the lab is below, please let me know if you have any further questions!



Two responses: The first is that the client should NOT use PVDF or any other nylon-based membranes. Chicken antibodies bind non-specifica...

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Vielen Dank für Ihre Antwort.


Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie besprochen, habe ich eine kostenlose Ersatzlieferung des ab97135 für Sie in Auftrag gegeben. Sie hat die Referenznummer 1212...

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Vielen dank für Ihre Antwort.

In diesem Fall würde ich Ihnen gerne anbieten nach dem Ausschlussverfahren vorzugehen, wenn Sie damit einverstanden sind.

Ich würde Ihnen gerne ein kostenloses Ersatzprodukt für den se...

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Vielen Dank für die Email und die Zusendung des ausgefüllten Fragebogens. Anja ist leider außer Haus. Wenn es Ihnen recht ist würde ich mich gerne in dieser Zeit um Ihren Fall kümmern.

Es tut mir Leid, dass sie Proble...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"