Goat Anti-Dog IgM H&L (HRP) (ab112835)
Key features and details
- Goat Anti-Dog IgM H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, WB, ELISA, IHC-P
Related conjugates and formulations
Overview
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Product name
Goat Anti-Dog IgM H&L (HRP)
See all IgM secondary antibodies -
Host species
Goat -
Target species
Dog -
Specificity
No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. ab112835 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/200 - 1/500.
|
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WB |
1/1000 - 1/30000. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution.
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|
ELISA |
1/10000 - 1/100000.
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|
IHC-P |
1/200 - 1/500.
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Notes |
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ICC/IF
1/200 - 1/500. |
WB
1/1000 - 1/30000. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution. |
ELISA
1/10000 - 1/100000. |
IHC-P
1/200 - 1/500. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab112835 has been referenced in 3 publications.
- Porcellato I et al. Comparison of immunohistochemical and qPCR methods from granulomatous dermatitis lesions for detection of leishmania in dogs living in endemic areas: a preliminary study. Parasit Vectors 15:104 (2022). PubMed: 35331318
- Yang J et al. Toxoplasma gondii and Neospora caninum Infections in Stray Cats and Dogs in the Qinghai-Tibetan Plateau Area, China. Animals (Basel) 12:N/A (2022). PubMed: 35681854
- Zhang J et al. Serological Analysis of IgG and IgM Antibodies against Anaplasma spp. in Various Animal Species of the Qinghai-Tibetan Plateau. Animals (Basel) 12:N/A (2022). PubMed: 36230463