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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed (ab6006)
Overview
Product name
Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed
See all IgG + IgM + IgA products (59) ...
Target species
Mouse
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c). This reagent is suitable for the detection of all mouse subclasses, isotypes and chain combinations.
Tested applications
ICC/IF, Dot Blot, WB, ELISA, Electron Microscopy, IHCmore details
Minimal
cross-reactivity
Humanmore details
Immunogen
Mouse IgG, IgA and IgM whole molecules
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
Conjugation notes
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons).
Clonality
Polyclonal
Isotype
IgG
Research areas
Secondary antibodies >> anti-Mouse >> IgG + IgM + IgA >> Enzyme >> HRP
Immunology >> Immunoglobulins >> Heavy Chain >> IgM
Immunology >> Immunoglobulins >> Heavy Chain >> IgA
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Western blot - Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed (ab6006)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/500 - 1/2500.ICC/IF: 1/500 - 1/2500.
Dot: Use at an assay dependent dilution.
WB: 1/2000 - 1/10000.
ELISA: 1/120000(This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.)
EM: Use at an assay dependent dilution.
IHC: Use at an assay dependent dilution.
Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed images:
Western blot - Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed (ab6006)
Ab6006 was used at dilution 1/3000 with the primary antibody ab2861 in WB. See the review on ab2861.
References for Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed (ab6006)
This product has been referenced in:
- Patel Pet al. The endogenous production of hydrogen sulphide in intrauterine tissues. Reprod Biol Endocrinol 7:10 (2009). WB.Read more (PubMed: 19200371) »
- Adinolfi Eet al. Expression of the P2X7 receptor increases the Ca2+ content of the endoplasmic reticulum, activates NFATc1, and protects from apoptosis. J Biol Chem 284:10120-8 (2009). WB.Read more (PubMed: 19204004) »
See all 2 publications for this product
Publishing research using ab6006? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"