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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgM
Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (PE) (ab5932)
Overview
Product name
Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (PE)
See all IgM products (217) ...
Target species
Mouse
Specificity
Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgM, and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or human, bovine or horse serum proteins.
Tested applications
ICC/IF, Immunomicroscopy, Flow Cytmore details
Immunogen
Mouse IgM mu heavy chain
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 15mg/ml BSA (IgG and protease free), 0.25M Sodium Chloride, 0.01M Sodium Phosphate. pH 7.6
Concentration
Concentration information loading...
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notes
R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Secondary antibodies >> anti-Mouse >> IgM >> Fluorophore >> Phycoerythrin
Immunology >> Immunoglobulins >> Heavy Chain >> IgM
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/100 - 1/250.ICC/IF: 1/100 - 1/250.
IM: Use at an assay dependent dilution. (As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications. )
Flow Cyt: 1/100 - 1/250.(The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.)
References for Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (PE) (ab5932)
This product has been referenced in:
- Lappalainen Jet al. A protocol for generating high numbers of mature and functional human mast cells from peripheral blood. Clin Exp Allergy 37:1404-14 (2007). Flow Cyt; Mouse.Read more (PubMed: 17845422) »
See 1 publication for this product
Publishing research using ab5932? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"