Goat F(ab) Anti-Mouse IgG H&L (1.4nm Gold (ultra-small) + FITC) (ab33109)

Overview

  • Product nameGoat F(ab) Anti-Mouse IgG H&L (1.4nm Gold (ultra-small) + FITC)See all IgG secondary antibodies ...
  • Description
    Goat F(ab) polyclonal Secondary Antibody to Mouse IgG (1.4nm Gold (ultra-small) + FITC)
  • Target speciesMouse
  • Tested applicationsWB, Electron Microscopy, ICC/IF more details
  • Immunogen

    Mouse IgG whole molecule.

  • ConjugationFITC + Gold 1.4nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 0.1% BSA, 20mM PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesChromatographically purified through gel filtration columns.
  • Clonality Polyclonal
  • IsotypeIgG
  • General notes

     

    This product is a revolutionary new immunocytochemical reagent. This unique antibody probe contains a molecular label which contains both the 1.4nm ultra-small gold particle and fluorescein, enabling both fluorescence and electron microscope observation of a sample stained in a single labelling procedure. This probe is smaller than a whole IgG molecule, does not aggregate, and fluorescence quenching due to the goold particle is negligible. Normal methologies may be used successfully with this product. Due to some quenching of fluorescence by the gold particle, slightly higher concentrations of antibody are recommended for incubations. A blocking agent of 5% non-fat dried milk has been found to reduce background in some cases: this should be used before incubation with probe (in standard wash/blocking steps), and additionally, the Fluorescein-ultra-small gold probe should be diluted in a solution also containing 5% non-fat dried milk before it is applied.

  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab33109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
Electron Microscopy
ICC/IF
  • Application notesElectron Microscopy: Use at an assay dependent dilution.
    IF: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat F(ab) Anti-Mouse IgG H&L (1.4nm Gold (ultra-small) + FITC) (ab33109)

    ab33109 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your call yesterday and for your patience while I have been in touch with the lab regarding your enquiries.

    This is not very clear on the datasheet of ab33109, but this is actually a Fab' fragment conjugated to both ultra small ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"