Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesFluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)
Absorption Wavelength: 495 nm Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 5.0 moles FITC per mole of Goat IgG Fab
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Goat F(ab) Anti-Mouse IgG H&L (FITC) (ab6669)
This product has been referenced in:
Mogilenko DA et al. Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages. FASEB J : (2012).
Read more (PubMed: 22271762) »
Mogilenko DA et al. Modified low density lipoprotein stimulates complement C3 expression and secretion via liver X receptor and toll-like receptor 4 activation in human macrophages. J Biol Chem : (2011).
Read more (PubMed: 22194611) »