Overview

  • Product name
    Goat F(ab) Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. It was tested against anti goat F(c) fragment to verify no contaminating goat F(c) was present in the fraction. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
  • Tested applications
    Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • Conjugation notes
    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/500 - 1/2500.
ELISA 1/10000 - 1/100000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

Images

  • Ab6823 was used with the primary antibody ab15263 in IHC-P. See Abreview on ab15263.

References

This product has been referenced in:
  • Terraf P  et al. Tissue-Engineered Regeneration of Hemisected Spinal Cord Using Human Endometrial Stem Cells, Poly e-Caprolactone Scaffolds, and Crocin as a Neuroprotective Agent. Mol Neurobiol N/A:N/A (2016). Read more (PubMed: 27624387) »
  • Wilson K  et al. Bacterial expression of human kynurenine 3-monooxygenase: solubility, activity, purification. Protein Expr Purif 95:96-103 (2014). WB . Read more (PubMed: 24316190) »

See all 6 Publications for this product

Customer reviews and Q&As

The product you are looking for is ab6668, Goat F(ab) polyclonal Secondary Antibody to Mouse IgG without HRP.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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