Goat F(ab) Anti-Rabbit IgG H&L (1.4nm Gold (ultra-small) + FITC) (ab33110)

Overview

  • Product nameGoat F(ab) Anti-Rabbit IgG H&L (1.4nm Gold (ultra-small) + FITC)See all IgG secondary antibodies ...
  • Description
    Goat F(ab) polyclonal Secondary Antibody to Rabbit IgG (1.4nm Gold (ultra-small) + FITC)
  • Target speciesRabbit
  • Tested applicationsElectron Microscopy, ICC/IF, WBmore details
  • Immunogen

    Rabbit IgG whole molecule.

  • ConjugationFITC + Gold 1.4nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 0.1% BSA, 20mM PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesChromatographically purified through gel filtration columns.
  • Clonality Polyclonal
  • IsotypeIgG
  • General notes

     

    This product is a revolutionary new immunocytochemical reagent. This unique antibody probe contains a molecular label which contains both the 1.4nm ultra-small gold particle and fluorescein, enabling both fluorescence and electron microscope observation of a sample stained in a single labelling procedure. This probe is smaller than a whole IgG molecule, does not aggregate, and fluorescence quenching due to the goold particle is negligible. Normal methologies may be used successfully with this product. Due to some quenching of fluorescence by the gold particle, slightly higher concentrations of antibody are recommended for incubations. A blocking agent of 5% non-fat dried milk has been found to reduce background in some cases: this should be used before incubation with probe (in standard wash/blocking steps), and additionally, the Fluorescein-ultra-small gold probe should be diluted in a solution also containing 5% non-fat dried milk before it is applied.

  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab33110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
ICC/IF
WB
  • Application notesElectron Microscopy: Use at an assay dependent dilution.
    IF: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat F(ab) Anti-Rabbit IgG H&L (1.4nm Gold (ultra-small) + FITC) (ab33110)

    ab33110 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab33110.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"