Goat F(ab) Anti-Rabbit IgG H&L (Biotin) (ab7055)

Overview

  • Product nameGoat F(ab) Anti-Rabbit IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
  • Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
  • Immunogen

    Rabbit IgG whole molecule

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG Fab molecule
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Dot blot
WB
ELISA
IHC
Immunomicroscopy
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: 1/50000 - 1/250000.
    IHC: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/50000 to 1/250000 of the reconstitution concentration is suggested for this product.

    Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat F(ab) Anti-Rabbit IgG H&L (Biotin) images

    • Ab7055 was used at dilution 1/200 with the primary antibody ab32090 in IHC-P. See the review on ab32090.

    References for Goat F(ab) Anti-Rabbit IgG H&L (Biotin) (ab7055)

    ab7055 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"