Goat F(ab) Anti-Rabbit IgG H&L (HRP) (ab7171)
Key features and details
- Goat F(ab) Anti-Rabbit IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC
Related conjugates and formulations
Overview
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Product name
Goat F(ab) Anti-Rabbit IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c). -
Tested applications
Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
Immunogen
Rabbit IgG whole molecule
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
IgG and protease free -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. -
Conjugation notes
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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Dot blot |
Use at an assay dependent dilution.
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WB |
1/1000 - 1/5000.
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ELISA |
1/10000 - 1/50000.
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Electron Microscopy |
Use at an assay dependent dilution.
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IHC |
Use at an assay dependent dilution.
|
Notes |
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ICC/IF
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
WB
1/1000 - 1/5000. |
ELISA
1/10000 - 1/50000. |
Electron Microscopy
Use at an assay dependent dilution. |
IHC
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (15)
ab7171 has been referenced in 15 publications.
- Zhang SQ et al. APOBEC3B expression has prognostic significance in cervical cancer. Int J Clin Exp Pathol 16:48-56 (2023). PubMed: 37033395
- Fan R et al. Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male Rats. Evid Based Complement Alternat Med 2022:2112359 (2022). PubMed: 35432561
- Shaw IW et al. A role for steroid 5 alpha-reductase 1 in vascular remodeling during endometrial decidualization. Front Endocrinol (Lausanne) 13:1027164 (2022). PubMed: 36465608
- Nikiforova N et al. CM-Dil Staining and SEC of Plasma as an Approach to Increase Sensitivity of Extracellular Nanovesicles Quantification by Bead-Assisted Flow Cytometry. Membranes (Basel) 11:N/A (2021). PubMed: 34357175
- Guan Y et al. Protective multi-target effects of DL-3-n-butylphthalide combined with 3-methyl-1-phenyl-2-pyrazolin-5-one in mice with ischemic stroke. Mol Med Rep 24:N/A (2021). PubMed: 34643246