Goat F(ab) Anti-Rat IgG H&L (HRP) (ab102191)

Overview

  • Product name
    Goat F(ab) Anti-Rat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target species
    Rat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • Tested applications
    Suitable for: ELISA, WB, ICC, IHC-Pmore details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab) fragments were generated using a papain digestion. F(ab')2, Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to HRP.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/100000. Primary dilution.
WB 1/5000 - 1/50000. Colorimetric: Use 1/5,000-1/30,000 dilution; Chemiluminescent: Use 1/10,000-1/50,000 dilution.
ICC 1/200 - 1/5000.
IHC-P 1/200 - 1/5000.

References

ab102191 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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