Overview

  • Product nameGoat F(ab) Anti-Rat IgG H&L (HRP)See all IgG secondary antibodies ...
  • Description
    Goat F(ab) polyclonal Secondary Antibody to Rat IgG - H&L (HRP)
  • Target speciesRat
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
  • Tested applicationsICC/IF, WB, ELISA, Electron Microscopy, IHC, Dot Blotmore details
  • Immunogen

    Rat IgG whole molecule

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • Conjugation notesHorseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
WB Use at an assay dependent dilution.
ELISA 1/10000 - 1/40000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.
Dot Blot Use at an assay dependent dilution.

References for Goat F(ab) Anti-Rat IgG H&L (HRP) (ab7177)

ab7177 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"